“I discovered, that the saline solution which I had used had not been prepared with distilled water, but with pipe water supplied by the New River Water Company. As this water contains minute traces of various inorganic substances, I at once tested the action of saline solution made with distilled water and I found that I did not get the effects described in the paper referred to. It is obvious therefore, that the effects I had obtained are due to some of the inorganic constituents of the pipe water”
These were the words of Sydney Ringer, a British clinician and physiologist, is recognised for formulating the 'Ringer's solution,' which facilitated the maintenance of isolated frog heart contractions, ultimately contributing to the creation of an isotonic solution utilised in medical research.
Which particular inorganic compound referred to here had the most profound impact in maintaining the frog heart contractions?